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Waters recommends pre-slit PTFE/silicone septa, for venting and accurate sample draw. They also reduce the possibility of septum coring in bottom-draw needles. For applications with a volatile solvent that require non-slit septa, there are simple steps you can take to reduce creating a vacuum. Do not fill the vial; leave headspace.
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Quality vials are essential for reliable analytical results. All Waters Certified Vials are tested by LC or LC/MS to assure quality and cleanliness. The test results confirm the vials, cap and septa have been properly manufactured, handled and packaged, and are free from any potential contaminants which could result in chromatographic ghost peaks.
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