Compatibility of septa with buffers in HPLC

5.9 - 6.9, 8.8 - 9.8. 200. Ammonia / ammonium hydroxide. 8.2 – 10.2. 200. Modern HPLC method development is dominated by a small number of pH adjusting reagents and/or buffers, that are prevalent even when the method uses UV detection. This is driven primarily by the requi.

PTFE/Silicone septa are ideal for use in most HPLC and GC applications where resealability and purity are critical. Pre-slit PTFE/Silicone Septa Pre-slit septa are offered in many of the same formulations as for non-slit PTFE/silicone septa and shares most of the physical and chemical characteristics.

Buffers are solutions of a weak acid and its conjugate base, or a weak base and its conjugate acid. In analytical chemistry, buffers are typically used in reversed-phase high performance liquid chromatography (RP-HPLC), when the sample contains acidic or basic functional groups. Buffers mitigate the influence of hydrogen/hydronium and hydroxide

Sep 20, 2017 · This month we take a look at the important topic of buffer choice for HPLC separations, how to the choose the correct buffer type and concentration as well as how to avoid variability in retention and selectivity. Buffer — a solution that resists a change in pH when small amounts of acid or alkali are added to it, or when it is diluted with

Aug 22, 2023 · Compatibility Check: Ensure material compatibility with your sample solvents and instrument requirements. Optimal Septa Selection. Precision in septa selection is paramount: Material Matchup: Choose from silicone, rubber, or inert PTFE. PTFE septa are excellent for samples sensitive to contamination.

Using Buffers with HPLC and LC-MS When samples contain ionizable compounds, the mobile phase pH can be one of the most important variables in the control of retention in a reversed-phase HPLC (RP-HPLC) separation. However, if it is not controlled properly, pH can be a source of many problems.

compatibility, effective sealing/resealing and low penetration force make PTFE/silicone septa suitable for all types of chromatographic applications. A thin film of PTFE is laminated to the side of the septum that faces the sample to limit exposure of the elastomer to the solvent. PTFE/Silicone septa are ideal for use in most HPLC and

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0.1% formic acid, not 1%) that is compatible with the chromatography. b. Use the highest quality of additives available. c. Use additives (for example, formic acid) that have low concentrations of iron and other metal ions. Acetic acid can contain a significant amount of iron and other metal ions.1 d.

Autosampler Vials & Caps for HPLC & GC›. Septa Selection Tool. Septa Selection for Autosampler Vials. Use this simple selector tool to choose the right SureSTART septum, cap, and vial to prevent autosampler injection failure. Go to SureSTART Selection Guide.

• Pre-slit septa perform better than solid septa with ACQUITY UPLC and H-Class systems. • Crimp caps are not recommended for use with the Sample Organizer because the caps' reflective surface can inhibit shelf recognition. 700002644, 30 L PEEK 205000369, 30 L FEP with SS tip 205000362, 30 L SS with SS tip 205000363, 15 L SS with SS tip

compatibility, effective sealing/resealing and low penetration force make PTFE/silicone septa suitable for all types of chromatographic applications. A thin film of PTFE is laminated to the side of the septum that faces the sample to limit exposure of the elastomer to the solvent. PTFE/Silicone septa are ideal for use in most HPLC and

Buffers are solutions of a weak acid and its conjugate base, or a weak base and its conjugate acid. In analytical chemistry, buffers are typically used in reversed-phase high performance liquid chromatography (RP-HPLC), when the sample contains acidic or basic functional groups. Buffers mitigate the influence of hydrogen/hydronium and hydroxide

Solutions for Preparative HPLC - Application Compendium. Application compendium for preparative LC. Optimize Binary Pump. Optimization of LC and LC/MS. HPLC Separation Fundamentals. Basics of LC separation. New to HPLC Tips for Avoiding Beginner Pitfalls. Essentials for new LC users. LC Troubleshooting Series Retention Time Shifts

Septa chemical compatibility. Solvent PTFE PTFE/Silicone PTFE/Silicone/PTFE* PTFE/Red rubber Fluoroelastomer PTFE/Butyl Acetonitrile Hydrocarbons (hexane, heptane, methane) Methanol Benzene THF Toluene DMF DMSO Ether Chlorinated solvents (methylene chloride) Alcohols (ethanol) Acetic acid Acetone Phenol Cyclohexane